These diagrams show the differences in quality of RNA obtained from the different fractions.High RIN values (around 8.0) were consistently observed for total RNA from SC (Figure 1B) despite the fact that the milk sample was kept on ice for three hour until transported to the laboratory for RNA extraction. The RNA from MFG contains low molecular weight fragments and a very low amount of ribosomal RNA (RIN = 6.0) (Figure 1E).The membrane of MFG contains specific components such as phospholipids, proteins and glycophospholipids that are known to have an affinity for the surface of bacterial cells. By analyzing all the MFG reads not mapping to the bovine genome (11 million reads), we identified the presence of 618 different bacteria.
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